PMC

(A) Western blot to detect Maid in total protein isolated from the indicated tissues of adult zebrafish. Ponseau-S staining shows total protein bands. (B) Top: Hematoxylin and eosin (HE) staining of the whole body and liver of a WT zebrafish embryo at 30 dpf, and the liver of a WT adult zebrafish at 6 months of age. Bottom: Immunohistochemical staining to detect Maid in the zebrafish tissues in the top panel. Black arrow indicates the liver, where the highest level of Maid expression was observed. (C) Top: HE staining of zebrafish kidney at 6 months of age. Bottom: Immunohistochemical staining to detect Maid in the zebrafish kidney in the top panel. (D) Localization of Maid in zebrafish liver. Immunofluorescent analysis of Maid protein localization in untreated juvenile zebrafish liver, adult zebrafish liver, and a DEN-induced foci. Red, anti-zebrafish Maid antibody; blue, DAPI (visualization of nuclei).

(A) Western blot to detect Maid in total protein isolated from the indicated tissues of adult zebrafish. Ponseau-S staining shows total protein bands. (B) Top: Hematoxylin and eosin (HE) staining of the whole body and liver of a WT zebrafish embryo at 30 dpf, and the liver of a WT adult zebrafish at 6 months of age. Bottom: Immunohistochemical staining to detect Maid in the zebrafish tissues in the top panel. Black arrow indicates the liver, where the highest level of Maid expression was observed. (C) Top: HE staining of zebrafish kidney at 6 months of age. Bottom: Immunohistochemical staining to detect Maid in the zebrafish kidney in the top panel. (D) Localization of Maid in zebrafish liver. Immunofluorescent analysis of Maid protein localization in untreated juvenile zebrafish liver, adult zebrafish liver, and a DEN-induced foci. Red, anti-zebrafish Maid antibody; blue, DAPI (visualization of nuclei).

MAiD Care Ecology Model. Community Partner Supports: advocate for/facilitate MAiD care across hospital/community/region; local and national research, policy-making, practice standards and education collaborations. Organization Supports: infrastructure and resources to enable sustainable, accountable, high-quality MAiD hospital services (forms, protocols, leader support, etc.). MAiD Team Supports: interprofessional MAiD service provides whole-person care to patient and family; care coordination; structures enable team member engagement/resilience/development. Clinical Team Supports: facilitate referrals of patient requests for MAiD; support collaboration with ADRAS to enhance patient quality of life and explore all care options. Family Supports: education on MAiD process; emotional support; “death choreography”; bereavement support. Patient Supports: timely access to MAiD services using narrative, patient-centred approach; honoring the emotional, social, spiritual, and physical needs of the patient through the assessment and dying process

The graph depicts the ages of patients at initial presentation to the MAID clinic. MAID, Multiple Autoimmunity and Immunodeficiency Clinic